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Mouse Thrombin Assay and ELISA Kits

Mouse TAT Complexes ELISA Kit

Catalog No.: IRAPKT175

$495

Category: Assay and ELISA Kits

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Product Description

Mouse TAT Complexes ELISA Kit


Mouse TAT Complexes ELISA Kit applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TAT. Standards or samples are then added to the microtiter plate wells and TAT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TAT present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TAT are added to each well to sandwich the TAT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain TAT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.

(4-12-16)

Factor XI Deficiency Alters the Cytokine Response and Activation of Contact Proteases during Polymicrobial Sepsis in Mice


CE Bane Jr, I Ivanov, A Matafonov, KL Boyd, Q Cheng - PloS one, 2016
... ... system (Luminex Corporation, Austin, TX). Mouse specific ELISAs were used to measure plasma levels of serum amyloid P (SAP), thrombin-antithrombin (TAT) complex and fibrinogen (all from Innovative Research, Novi, MI). ...

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Mouse TAT Complexes ELISA Kit

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