Mouse Prothrombin Total ELISA Kit
This mouse prothrombin/thrombin total antigen assay is intended for the quantitative determination of total prothrombin and thrombin in mouse plasma, serum, tissue extracts, and cell culture media. Mouse prothrombin, thrombin, and thrombin-antithrombin complex will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of prothrombin in the sample.