Mouse Prolactin ELISA Kit
Mouse prolactin (PRL) is a 197 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk.
This mouse prolactin antigen assay is intended for the quantitative determination of prolactin antigen in mouse plasma. The assay measures total mouse prolactin in the 0.5-500ng/ml range. Samples with prolactin levels above 500ng/ml should be diluted in blocking buffer before use. Use of undiluted or 1:1 diluted normal plasma is suggested to ensure that resulting values fall within the linear range of the assay. Optimal dilutions should be experimentally determined by the researcher. Strip well format. Reagents for up to 96 tests.
Mouse prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, anti-mouse prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development which is measured at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prolactin. Color development is proportional to the concentration of prolactin in the samples.
Detection Target: Prolactin
Range: 0.5 - 500 ng/ml
Sensitivity: 0.253 ng/ml
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