Mouse Plasminogen Assay and ELISA Kits

Mouse Plasminogen Total ELISA Kit

Catalog No.: IMPLGKT-TOT


Category: Assay and ELISA Kits

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Product Description

Mouse Plasminogen Total ELISA Kit

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis.

This mouse plasminogen total assay is for the quantitative determination of total plasminogen and plasmin in mouse plasma, serum, urine, cell culture media, or tissue extracts. The assay measures plasminogen and plasmin antigens in the 1 - 500 ng/ml range. If the unknown is thought to have high plasminogen/plasmin levels, dilutions may be made in a similar biological fluid devoid of plasminogen or in blocking buffer. A 1:10,000 dilution for normal mouse plasma is suggested for best results. A 1:50 dilution for mouse urine is suggested for best results. Strip well format. Reagents for up to 96 tests.

Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-mouse plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.

Detection Target: Plasminogen
Reactivity: Mouse
Range: 1 - 500 ng/ml
Sensitivity: 0.28 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Neutralization of the antifibrinolytic function of plasminogen activator inhibitor1 resolves skin fibrosis

R Lemaire, T Burwell, H Sun, T Delaney, J Bakken - Arthritis & Rheumatology, 2015
... Mouse active PAI-1 and active plasmin levels were determined in plasma and skin lysates using ELISA kits as instructed by manufacturer (Innovative Research (Novi, Michigan). RNA analysis. Murine skin tissues were homogenized using a Polytron (Kinematica, Bohemia, ...

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Mouse Plasminogen Total ELISA Kit

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