Mouse Leptin ELISA Kit
Mouse Leptin ELISA kit is designed for detection of mouse leptin in plasma,serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures leptin in less than 5 hours. A polyclonal antibody specific for leptin has been pre-coated onto a microplate. Leptin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for leptin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.