Mouse IGF-1 ELISA Kit
Mouse IGF-1 ELISA kit is designed for detection of mouse IGF-1 in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IGF-1 in less than 5 hours. A polyclonal antibody specific for mouse IGF-1 has been pre-coated onto a microplate. Mouse IGF-1 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for mouse IGF-1, which is recognized by a streptavidin peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
HTML Glucocorticoid Receptor Knockdown Decreases the Antioxidant Protection of B16 Melanoma Cells: An Endocrine System-Related Mechanism that CompromisesE Obrador, SL Valles, M Benlloch, JA Sirerol - PloS one, 2014
...Concentration of IL-6 in the serum was determined using commercially available mouse cytokine ELISA kits from Innovative Research (Novi, MI). Glucocorticoid receptor knockdown: lentivirus production, titering, and transduction of target cell lines ...