Mouse IL-2 ELISA Kit
Mouse IL-2 (aka TCGF) is a 149 amino acid 15 kDa disulfide-linked monomeric glycosylate Type I cytokine 1. IL-2 induces proliferation of a variety of lymphocytes including CD4 and CD8 T cells, natural killer cells, and B cells. Serum IL-2 levels are elevated in several cancers and autoimmune conditions and lymphocyte secretion is decreased in HIV patients. IL-2 binds to the IL-2R receptor which is composed of three subunits of varying affinity.
This mouse Interleukin-2 (IL-2) antigen assay is intended for the quantitative determination of total mouse IL-2 antigen in plasma, serum, cell culture media, or other biological fluids. The assay measures mouse IL-2 antigen in the 0.01 - 10 ng/ml range. If the unknown is thought to have high IL-2 levels, dilutions may be made in culture media or in blocking buffer. Strip well format. Reagents for up to 96 tests.
Mouse IL-2 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IL-2. The amount of color development is proportional to the concentration of mouse IL-2 in the samples.
Detection Target: Interleukin 2
Range: 0.01 - 10 ng/ml
Sensitivity: 0.00085 ug/ml
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