Mouse IgG Assay and ELISA Kits

Mouse IgG ELISA Kit

Catalog No.: IMSIGGKT


Category: Assay and ELISA Kits

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Product Description

Mouse IgG ELISA Kit

IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

This Mouse Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total mouse IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. This assay does not distinguish IgG subclasses. Strip well format. Reagents for up to 96 tests. The assay measures IgG antigen in the 0.5-500 ng/ml range. If the unknown is thought to have high IgG levels, dilutions may be made in diluent. A 1:1,000,000 dilution for normal mouse plasma is suggested for best results. A 1:5,000 minimum dilution for cell culture supernate is suggested for best results.

Mouse IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-mouse IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IgG. Color development is directly proportional to the concentration of total IgG in the samples.

Detection Target: IgG
Reactivity: Mouse
Range: 0.5 - 500 ng/ml
Sensitivity: 0.119 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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Total serum IgG, IgG2b, and anti-chromatin antibodies (with respect to anti-nucleosome antibody expression) were analyzed using commercially available Mouse IgG (Innovative Research, Novi, MI), Mouse IgG2b (Abcam, Cambridge, MA) and QUANTA Lite Chromatin (Inova

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Mouse IgG ELISA Kit

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