Mouse IgE Assay and ELISA Kits

Mouse IgE ELISA Kit

Catalog No.: IMSIGEKT


Category: Assay and ELISA Kits

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Product Description

Mouse IgE ELISA Kit

In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Involvement of Corneal Lymphangiogenesis in a Mouse Model of Allergic Eye Disease Corneal Lymphangiogenesis in Ocular Allergy

HS Lee, D Hos, T Blanco, F Bock, NJ Reyes, R Mathew - Ophthalmology & Visual , 2015
... 7, and serum was collected as previously described. 37 Total IgE was measured via ELISA, as per manufacturer's instructions (Innovative Research, Novi, MI, USA). In Vitro T-Cell Assay. This has been previously described.

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Mouse IgE ELISA Kit

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