Mouse IgA Assay and ELISA Kits

Mouse IgA ELISA Kit

Catalog No.: IMSIGAKT


Category: Assay and ELISA Kits

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Product Description

Mouse IgA ELISA Kit

IgA is the main immunoglobulin in mucous secretions and the second most abundant immunoglobulin in serum. It defends against inhaled and ingested pathogens by binding an Fc receptor on myeloid leukocytes. The equivalent mouse receptor has not yet been identified. Human IgA has two distinct subclasses and is monomeric in serum while mouse IgA does not have subclasses and is dimeric in serum.

This mouse immunoglobulin A (IgA) antigen assay is intended for the quantitative determination of total mouse IgA antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay measures IgA antigen in the 0.1 - 100 ng/ml range. If the unknown is thought to have high IgA levels, dilutions may be made in blocking buffer. A 1:200,000 dilution for normal mouse plasma is suggested for best results.Strip well format. Reagents for up to 96 tests.

Mouse IgA will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-mouse IgA antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IgA. Color development is directly proportional to the concentration of total IgA in the samples.

Detection Target: IgA
Reactivity: Mouse
Range: 0.1 - 100 ng/ml
Sensitivity: 0.074 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Neonatal Phytoestrogen Exposure Alters Oviduct Mucosal Immune Response to Pregnancy and Affects Preimplantation Embryo Development in the Mouse 1

WN Jefferson, E Padilla-Banks, JY Phelps... - Biology of ..., 2012 - BioOne
...the ELISA. The non-flushed oviduct was pulverized on dry ice and then homogenized in 200 l of 1 diluent supplied in the mouse immunoglobulin A (IgA) ELISA kit (Innovative Research, Novi, Michigan). Samples were centrifuged...

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Mouse IgA ELISA Kit

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