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Mouse Fibrinogen Assay and ELISA Kits

Mouse Fibrinogen Total ELISA Kit

Catalog No.: IMFBGNKT

$295

Category: Assay and ELISA Kits

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Product Description

Mouse Fibrinogen Total ELISA Kit


Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two- to four-hundred-fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis.

This mouse fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in mouse plasma and serum. The assay measures total mouse fibrinogen in the 3.125-800 ng/ml range. If the unknown is thought to have high fibrinogen levels, dilutions may be made in diluent. A 1:100,000-1:400,000 dilution for normal mouse plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.

Mouse fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

Detection Target: Fibrinogen
Reactivity: Mouse
Range: 3.125 - 800 ng/ml
Sensitivity: 0.241 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

(4-12-16)

Factor XI Deficiency Alters the Cytokine Response and Activation of Contact Proteases during Polymicrobial Sepsis in Mice


CE Bane Jr, I Ivanov, A Matafonov, KL Boyd, Q Cheng - PloS one, 2016
... ... system (Luminex Corporation, Austin, TX). Mouse specific ELISAs were used to measure plasma levels of serum amyloid P (SAP), thrombin-antithrombin (TAT) complex and fibrinogen (all from Innovative Research, Novi, MI)....

(10-13-15)

FDP-E induces adipocyte inflammation and suppresses insulin-stimulated glucose disposal: Effect of inflammation and obesity on fibrinogen B mRNA.

M Kang, RA Vaughan, CM Paton - American Journal of Physiology-Cell Physiology, 2015
... Mouse fibrinogen ELISA was from Innovative Research (IMFBGNKT, Novi, MI). Bovine 101 insulin and 2-deoxy-D-glucose were from Sigma (St. ...

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Mouse Fibrinogen Total ELISA Kit

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