Mouse Hageman Factor Antigen Assay

Mouse Factor XII Total ELISA Kit



Category: Assay and ELISA Kits

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Mouse Factor XII Total ELISA Kit

Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a 'severe defect' in thrombus formation.

This mouse coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII antigen in mouse plasma. The assay measures Factor XII antigen in the 0.01 - 10 ng/ml range. If the unknown is thought to have high Factor XII levels, dilutions may be made in blocking buffer. A 1:50,000-1:400,000 dilution for normal mouse plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.

Mouse Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse Factor XII. Color development is proportional to the concentration of Factor XII in the samples.

Detection Target: Factor XII
Reactivity: Mouse
Range: 0.01 - 10 ng/ml
Sensitivity: 0.005 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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Mouse Factor XII Total ELISA Kit

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