Mouse Factor X ELISA Kit
Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X serves as the intersection of the intrinsic and extrinsic coagulation cascades and can be activated by either the extrinsic Factor VIIa / Tissue Factor compex or the intrinsic Factor IXa / Factor VIIIa complex. Factor Xa converts prothrombin to thrombin and is quickly inhibited by Antithrombin III in the presence of heparin.
This mouse Factor X total assay is for the quantitative determination of total Factor X and Xa in biological fluids. The assay measures Factor X antigen in the 2.5-500 ng/ml range. If the unknown is thought to have high Factor X levels, dilutions may be made in blocking buffer. A 1:1,000-1:2,000 dilution for normal mouse plasma or serum is suggested for best results. Strip well format. Reagents for up to 96 tests.
Mouse Factor X will bind to the affinity purified capture antibody coated on the microtiter plate. Factor X, Xa, and Xa in complex with inhibitors will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse Factor X primary antibody binds to the Factor X. Excess antibody is washed away and bound polyclonal antibody is then reacted with Streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total Factor X in the sample.
Detection Target: Factor X
Range: 2.5 - 500 ng/ml
Sensitivity: 0.146 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.