Mouse CRP ELISA Kit
CRP is an acute phase reactant which is elevated in plasma in response to increased interleukin-6 induced by inflammation, infection and tissue injury. CRP is expressed mainly in the liver and activates the complement pathway following calcium-dependent binding to phosphocholine on apoptotic, necrotic and microbial cells.
This mouse C-reactive protein (CRP) antigen assay is intended for the quantitative determination of total CRP in mouse plasma and other biological fluids. The assay measures CRP antigen in the 0.02 - 4 ng/ml range. Samples giving mouse CRP levels above 4 ng/ml should be diluted in blocking buffer before use. A 1:20,000 to 1:80,000 dilution for normal mouse plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Mouse CRP will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse CRP primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse CRP. Color development is proportional to the concentration of CRP in the samples.
Detection Target: C-Reactive Protein
Range: 0.02 - 4 ng/ml
Sensitivity: 0.01 ng/ml
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