Mouse alpha Macroglobulin ELISA Kit (Urine and Cell Culture Samples)
Mouse alpha Macroglobulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of mouse alpha macroglobulin in urine and cell culture. This assay employs a quantitative competitive enzyme immunoassay technique that measures mouse alpha macroglobulin in less than 3 hours. A polyclonal antibody specific for mouse alpha macroglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha macroglobulin in standards and samples is competed by a biotinylated alpha macroglobulin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. Alpha-2-Macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors (1). Increased serum alpha-2-Macroglobulin has been suggested to be associated with multiple sclerosis (MS)(2), glomerular disease (3), and with liver diseases (4). Mouse alpha-Macroglobulin (M-AMG) is believed to be a functional homologue of human alpha2-Macroglobulin (h-alpha 2M).