Mouse Antitrypsin Assay and ELISA Kits

Mouse Alpha-1 Antitrypsin ELISA Kit

Catalog No.: IRKTAH1147


Category: Assay and ELISA Kits

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Product Description

Mouse Alpha-1 Antitrypsin ELISA Kit

In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Rapid Renal Alpha-1 Antitrypsin Gene Induction in Experimental and Clinical Acute Kidney Injury

RA Zager, ACM Johnson, KB Frostad - PloS one, 2014
The renal cortical protein extracts and urine samples were assayed for mouse AAT by ELISA (Innovative Research, Inc., Novi, MI; Catalog IRKTAH1147). Cortical AAT samples were expressed as g/mg of extracted protein...

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Mouse Alpha-1 Antitrypsin ELISA Kit

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