Mouse Antiplasmin Total Antigen Assay ELISA kit
Alpha-2-antiplasmin is the major circulating inhibitor of plasmin. It plays a role in the regulation of intravascular fibrinolysis. Decreased levels of Alpha-2-antiplasmin may play an important role in the increased capacity of the fibrinolytic function and may be beneficial in the treatment of thrombotic diseases, acute pulmonary embolism, and hepatic repair.
This mouse alpha2-antiplasmin assay is for the quantitative determination of total alpha2-antiplasmin in mouse plasma. The assay measures alpha2-antiplasmin antigen in the 0.1-100 ng/ml range. If the unknown is thought to have high alpha2-antiplasmin levels, dilutions may be made in blocking buffer. A 1:10,000-1:20,000 dilution for normal mouse plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Total mouse alpha-2-antiplasmin will bind to the affinity purified capture antibody coated on the microtiter plate. Complexed and free alpha-2-antiplasmin will react with the antibody on the plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse alpha-2-antiplasmin primary antibody binds to the captured protein. Excess antibody is washed away and bound antibody is reacted with streptavidin conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse alpha-2-antiplasmin. Color development is proportional to the concentration of alpha-2-antiplasmin in the samples.
Detection Target: Alpha-2-antiplasmin
Range: 0.1 - 100 ng/ml
Sensitivity: 0.065 ng/ml
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