Mouse Albumin ELISA Kit
Mouse Albumin ELISA Kit -Albumin is a water-soluble protein with considerable structural stability which makes up 60 percent of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products. The sensitive quantitative measurement of total mouse albumin (murine serum albumin, MSA) in plasma, serum, urine or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. This kit has been validated for measurement of albumin in Balb-C mouse urine (33 ug/ml), nude mouse urine (19 ug/ml), and CD1 mouse plasma (29 mg/ml). Albumin is present in normal mouse plasma at a concentration of 20-30 mg/ml.
The assay measures mouse albumin in the 1-1000 ng/ml range. Samples giving mouse albumin levels above 1000ng/ml should be diluted in 1X diluent before use. A 1:500,000 to 1:1,000,000 dilution for normal plasma or a 1:1,000 dilution for urine is suggested for best results. Mouse albumin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-mouse albumin primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse albumin in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse albumin and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.