Mouse Albumin ELISA Kit
Albumin is a water-soluble protein with considerable structural stability which makes up 60% of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions, and medicinal products.
This mouse albumin antigen assay is intended for the quantitative determination of total albumin in mouse plasma, serum, urine & other biological fluids. The assay measures albumin antigen in the 1 - 1000 ng/ml range. If the unknown is thought to have high albumin levels, dilutions may be made in diluent. A 1:1,000,000-1:4,000,000 dilution for normal mouse plasma or a 1:1,000 dilution for normal mouse urine is suggested for best results. Strip well format. Reagents for up to 96 tests.
Mouse albumin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-mouse albumin antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse albumin. Color development is proportional to the concentration of albumin in the samples.
Detection Target: Albumin
Range: 1 - 1000 ng/ml
Sensitivity: 0.2 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Pharmaceutical Sciences) In The University of Michigan 2018.
...Albumin was assayed by ELISA (Innovative Research, Novi, MI) ... Cellular and biochemical analyses of BAL fluid to assess lung injury revealed that CFZ treatment reduced the LPS-induced BAL fluid cell count (Fig. 5C) and reduced the BAL fluid albumin concentrations (Fig. 5D).