Mouse Adiponectin ELISA Kit
Mouse Adiponectin ELISA Kit is designed for detection of adiponectin in mouse urine, plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures adiponectin in less than 4 hours. A polyclonal antibody specific for adiponectin has been pre-coated onto a microplate. Adiponectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for adiponectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Adiponectin, also known as Adipocyte Complement-Related Protein of 30kDa (Acrp30), is a secreted serum protein expressed exclusively in differentiated adipocytes. Studies indicated that decreased plasma adiponectin concentration is associated with obesity, insulin resistance (1), essential hypertension (2), inflammation and atherosclerosis (3), and acute myocardial infarction (4). On the other hand, increased adiponectin level leads to nephrotic syndrome (5 - 6).