Monkey Cardiac FABP ELISA Kit
The Monkey Cardiac FABP ELISA Kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses a mouse monoclonal anti-H-FABP antibody for solid phase (microtiter wells) immobilization and a different mouse monoclonal anti-H-FABP antibody conjugated to horseradish peroxidase (HRP) for detection. Standards and diluted samples are incubated with the HRP conjugate in the microtiter wells for 60 minutes. This results in H-FABP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of HFABP is proportional to optical density. H-FABP concentrations are determined by reference to a standard curve.