Monkey Anti-Tetanus Toxoid IgG1 ELISA Kit
The Monkey Anti-Tetanus Toxoid IgG1 ELISA Kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses tetanus toxoid for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse monoclonal anti-monkey IgG1 antibody for detection. Serum or plasma samples are diluted and incubated in the microtiter wells for 60 minutes. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. Anti-tetanus toxoid IgG1 molecules are thus sandwiched between immobilized tetanus toxoid and the detection antibody conjugate. The wells are then washed to remove unbound HRP-labeled antibodies, andTMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of antitetanus toxoid IgG1 is proportional to the optical density of the test sample.