Human vWF ELISA Kit with Negative Control
Human vWF ELISA Kit with Negative Control is designed for detection of human VWF in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures VWF in less than 5 hours. A murine antibody specific for VWF has been pre-coated onto a microplate. Human VWF in standards and samples is sandwiched by the immobilized monoclonal antibody and biotinylated polyclonal antibody specific for VWF, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.