Human Vitronectin Total ELISA Kit
Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion. Vitronectin binds to glycosaminoglycans, collagen, plasminogen, and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stabilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1. Vitronectin may also be involved in the regulation of bone metabolism.
This human vitronectin total assay is for the quantitative determination of total vitronectin in samples of human plasma, serum, urine, cell culture media, or tissue extracts. The assay measures total vitronectin antigen in the 0.5-100 ng/ml range. If the unknown is thought to have high vitronectin levels, dilutions may be made in blocking buffer. A 1:50,000 to 1:100,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human vitronectin will bind to the capture antibody coated on the microtiter plate. Free and ligand bound enzyme will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human vitronectin primary antibody binds to the vitronectin. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total vitronectin in the sample.
Detection Target: Vitronectin
Range: 0.5 - 100 ng/ml
Sensitivity: 0.176 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.