Human Vaspin ELISA Kit
Human Vaspin ELISA Kit is a 3.5 - 4.5 hour solid phase ELISA designed to measure human vaspin in serum and plasma. It contains recombinant human vaspin and antibodies raised against this protein. It has been shown to accurately quantify recombinant human vaspin. Results obtained with naturally occurring vaspin samples showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that the immunoassay kit can be used to determine relative mass values for natural human vaspin.
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for vaspin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any vaspin present is bound by the immobilized antibody. After washing away any unbound substances, an antibody specific for vaspin is added to the wells. Following a wash to remove any unbound detection antibody reagent, an antirabbit IgG HRP conjugate is added to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells and color develops in proportion to the amount of vaspin bound in the initial step. The color development is stopped and the intensity of the color is measured.