Human uPA Total ELISA Kit
Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration.
Human uPA total antigen assay is intended for the quantitative determination of total urokinase plasminogen activator in human plasma, serum, urine, cell culture media, or tissue extracts. The assay measures uPA antigen in the 0.1 - 50 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer or similar biological fluid devoid of uPA. Strip well format. Reagents for up to 96 tests.
Human uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-human uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
Detection Target: Urokinase
Range: 0.1 - 50 ng/ml
Sensitivity: 0.019 ng/ml
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