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Human uPA Assay and ELISA Kits

Human uPA Total ELISA Kit

Catalog No.: IHUPAKT-TOT

$495

Category: Assay and ELISA Kits

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Product Description

Human uPA Total ELISA Kit


Urokinase plasminogen activator (uPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. It is also implicated in events related to cell invasion/migration.

Human uPA total antigen assay is intended for the quantitative determination of total urokinase plasminogen activator in human plasma, serum, urine, cell culture media, or tissue extracts. The assay measures uPA antigen in the 0.1 - 50 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer or similar biological fluid devoid of uPA. Strip well format. Reagents for up to 96 tests.

Human uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-human uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.

Detection Target: Urokinase
Reactivity: Human
Range: 0.1 - 50 ng/ml
Sensitivity: 0.019 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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Human uPA Total ELISA Kit

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