Human uPA Assay and ELISA Kits

Human uPA ELISA Kit

Catalog No.: IHUPAKT


Category: Assay and ELISA Kits

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Product Description

Human uPA ELISA Kit

Urokinase plasminogen activator, along with its receptor uPAR, initiates a proteolytic cascade that results in the conversion of plasminogen to plasmin. Clinical studies have indicated that high uPA levels may elevate the risk for tumor invasion and metastasis. Increased expression and activity can exert potent arthritogenic properties in rheumatoid arthritis patients. Increased uPA activity may be an implication for the pathophysiology of endometriosis.

Human uPA activity assay is intended for the quantitative determination of active urokinase plasminogen activator in human plasma and urine. The assay measures active uPA in the 0.1 - 50 ng/ml range. If the unknown is thought to have high uPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.

Functionally active uPA present in samples will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Inactive or complexed uPA will not bind to the plate and will not be detected. Unbound uPA samples are washed away and an anti-uPA primary antibody is added. Excess primary antibody is washed away and bound antibody is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active uPA in the sample.

Detection Target: Urokinase
Reactivity: Human
Range: 0.1 - 50 ng/ml
Sensitivity: 0.024 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Bestimmung und klinische Wertigkeit von u-PA, t-PA, PAI-1 und PAI-2

K Mengele, N Harbeck, U Reuning, V Magdolen..., 2005 - schattauer.de
Tab. 2a chromogenes Substrat (S-2444) Innovative Research Inc, (USA) Active uPA functional assay (IHUPAKT) Merck Biosciences GmbH, Bad Soden, vorher Calbiochem- Novabiochem(-Novagen) GmbH (D) EMD Biosciences Inc, San Diego (USA)...

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Human uPA ELISA Kit

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