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Human tPA Assay and ELISA Kits

Human tPA Total ELISA Kit

Catalog No.: IHTPAKT-TOT

$495

Category: Assay and ELISA Kits

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Product Description

Human tPA Total ELISA Kit


tPA is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration.

This human tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tPA in human plasma and other biological fluids. The assay measures tPA antigen in the 0.2 25 ng/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Plasma samples must be applied directly to the plate without dilution for best results. Strip well format. Reagents for up to 96 tests.

Human tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-human tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. Color development is proportional to the concentration of tPA in the samples.

Detection Target: TPA
Reactivity: Human
Range: 0.2 - 25 ng/ml
Sensitivity: 0.0140 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(3-5-15)

Pharmacokinetics and Pharmacodynamics of Tissue Plasminogen Activator Administered Through an External Ventricular Drain


AH Kramer, C Jenne, JK Holodinsky, S Todd - Neurocritical Care, 2015
Enzyme lined immunosorbent assay (ELISA) testing was used to measure TPA (Innovative Research, Novi, MI) and D-dimer concentrations. D-dimer is a fibrin Neurocrit Care 123 Page 3.

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Human tPA Total ELISA Kit

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