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Human tPA Assay and ELISA Kits

Human tPA ELISA Kit

Catalog No.: IHTPAKT

$495

Category: Assay and ELISA Kits

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Product Description

Human tPA ELISA Kit


tPA is a serine protease that catalyzes the activation of plasminogen to plasmin. Clinical studies have indicated that high tPA levels may increase the risk for thrombosis, whereas decreased levels may cause neuronal plasticity and degeneration.

This human tissue-type plasminogen activator (tPA) activity assay is intended for the quantitative determination of active tPA in human plasma and other biological fluids. The assay measures active tPA in the 0.01 1 IU/ml range. If the unknown is thought to have high tPA levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.

Functionally active tPA will form a covalent complex with biotinylated human PAI-1 which is bound to the avidin coated on the microtiter plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. The amount of color development is directly proportional to the concentration of active tPA in the sample.

Detection Target: TPA
Reactivity: Human
Range: 0.01 - 1 IU/ml
Sensitivity: 0.0035 IU/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(9-3-15)

Activated Protein C Prevents Postsurgical Adhesion Band Formation


P Dinarvand, SM Hassanian, H Weiler, AR Rezaie
... The ELISA kit for measuring the concentration of tissue plasminogen activator (tPA) was obtained from Innovative Research Inc. Page 5. 5 (Novi, MI). ... Innovations, Inc., Royal Oak, MI) was analyzed by a kinetic assay as described.8 Statistical analysis ...

(3-29-10)

Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD)


N Patel, N Sundaram, M Yang, C Madigan... - Journal of Biological ..., 2010 - ASBMB
...Tissue plasminogen activator (tPA) levels in plasma were determined using ELISA as per the manufacturer's instructions (Innovative Research, Novi, MI). Data are expressed as mean SE of three independent experiments with duplicate samples.

(6-30-09)

Long-term stability of recombinant tissue plasminogen activator at-80 C


G Shaw, M Sperling... - BMC research notes, 2009 - biomedcentral.com
...sample. The samples were assayed for tPA activity using an ELISA technique that measures active tPA using plasminogen activator inhibitor 1 (PAI-1) (Human tPA Activity Assay, IHTPAKT, Innovative Research, Novi, MI, USA). In...

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Human tPA ELISA Kit

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