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Human Cytokine Assay and ELISA Kits

Human TNF Alpha ELISA Kit

Catalog No.: IRKTAH2628

$415

Category: Assay and ELISA Kits

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Product Description

Human TNF alpha ELISA Kit


Our Human TNF alpha ELISA Kit is a sandwich ELISA intended for the quantitative determination of human TNF alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
(3-1-12)

Overexpression of cGMP-dependent protein kinase I (PKG-I) attenuates ischemia-reperfusion-induced kidney injury


Y Li, X Tong, H Maimaitiyiming... - American Journal of ..., 2012 - Am Physiological Soc
...cytokines including IL-1, TNF-a, IL-6, and plasminogen activator inhibitor (PAI)-1 in the kidney homogenates were determined by ELISA using kits purchased from eBiosciences (San Diego, CA) for IL-1, TNF-a, and IL-6 and from Innovative Research (Novi, MI) for PAI-1.

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Human TNF Alpha ELISA Kit

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