Human Testosterone ELISA Kit
Human Testosterone ELISA Kit is based upon competitive solid phase ELISA. The patient sample competes with enzyme-linked testosterone for a fixed and limited number of antibody binding sites. In the assay, the testosterone standard or samples sera are incubated with testosterone-HRP conjugate and anti-testosterone. In this solid-phase system, the antibody bound testosterone will remain on the well while unbound testosterone will be removed by washing. A color (blue) is developed when the substrate, TMB is mixed with the antibody bound testosterone-HRP conjugate. After a short incubation, the enzyme reaction is stopped (blue color turns yellow) and the intensity of the color (yellow)is measured using an ELISA plate reader. The color is inversely proportional tothe concentration of testosterone in the sample.