Human Serum Amyloid A ELISA Kit
Human Serum Amyloid A ELISA kit is designed for detection of human SAA in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures total SAA in less than 4 hours. A monoclonal antibody specific for SAA has been pre-coated onto a 96-well microplate with removable strips. SAA in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for SAA, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.