Human RBP ELISA Kit (Plasma and Serum)
Human RBP ELISA Kit is designed for detection of human RBP in plasma, serum and urine. This assay employs a quantitative competitive sandwich enzyme immunoassay technique that measures human RBP in less than 2.5 hours. A polyclonal antibody specific for human RBP has been pre-coated onto a 96-well microplate with removable strips. RBP in standards and samples is competed by a biotinylated RBP sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added.
Retinol-binding protein (RBP) is a transport protein that acts by solubilizing and protecting its labile ligands in aqueous spaces. It also has diverse and specific functions in regulating the disposition, metabolism, and activities of retinoids (1). Retinol-binding protein is the specific plasma carrier of retinol, and encharged of the vitamin transport from the liver to target cells (2).Lower serum RBP level associates with diarrhea (3). High level of RBP in urine could be a good indicator of renal damage (4), microvascular complications with type-2 diabetes mellitus (5).