Human RBP ELISA Kit (Urine, Milk, Cell Culture)
Human RBP ELISA kit is designed for detection of human RBP in urine, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures RBP in less than 4 hours. A polyclonal antibody specific for RBP has been pre-coated onto a microplate. RBP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for RBP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Retinol-binding protein (RBP) is a transport protein that acts by solubilizing and protecting its labile ligands in aqueous spaces. It also has diverse and specific functions in regulating the disposition, metabolism, and activities of retinoids (1). Retinol-binding protein is the specific plasma carrier of retinol, and encharged of the vitamin transport from the liver to target cells (2).Lower serum RBP level associates with diarrhea (3). High level of RBP in urine could be a good indicator of renal damage (4), microvascular complications with type-2 diabetes mellitus (5).