Human Prorenin ELISA Kit for Non Plasma Samples
Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.
Human prorenin assay is intended for the quantitative determination of prorenin in non-plasma samples including cell culture media, tissue extracts, or urine samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin. The assay measures prorenin antigen in the 0.02 - 10 ng/ml range. If the unknown is thought to have high prorenin levels, dilutions may be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Only prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prorenin. The amount of color development is directly proportional to the concentration of prorenin in the sample.
Detection Target: Prorenin
Range: 0.02 - 10 ng/ml
Sensitivity: 0.016 ng/ml
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Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cellsShinji Yamashita, Kazal Boron Biswas, A.H.M. Nurun Nabi, Tsutomu Nakagawa, Fumiaki Suzuki, Akio Ebihara.
Biomedical Reports: Published online on July 5, 2018
"... The concentration of purified prorenin was measured using a human prorenin enzyme-linked immunosorbent assay (ELISA) kit (cat no. IHPRENKT-NP; Innovative Research, Inc., Novi, MI, USA). Prior to experimentation, purified prorenin was incubated at 37C for 1 h (23) to avoid cryo-activation due to preservation (24) ..."