Human Renin Assay and ELISA Kits

Human Prorenin ELISA Kit

Catalog No.: IHPRENKT


Category: Assay and ELISA Kits

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Product Description

Human Prorenin ELISA Kit

Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects.

Human prorenin assay is intended for the quantitative determination of prorenin in plasma samples and serum. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin. The assay measures human prorenin antigen in the 0.02 - 10 ng/ml range. If the unknown is thought to have high prorenin levels, dilutions should be made in plasma devoid of prorenin (cat IHPLA-SC-PREN) or in additional 0ng standard

Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Only prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prorenin. The amount of color development is directly proportional to the concentration of prorenin in the sample.

Detection Target: Prorenin
Reactivity: Human
Range: 0.02 - 10 ng/ml
Sensitivity: 0.013 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Prognostic value of renin and prorenin in heart failure patients with decreased kidney function

MK Szymanski, K Damman, DJ van Veldhuisen... - American Heart ..., 2011 - Elsevier
...Prorenin was measured with Human Prorenin ELISA kit (Innovative Research, Novi, MI) with prorenin-depleted human plasma and prorenin-depleted human plasma spiked with recombinant human prorenin from human embryonic kidney cell culture used as standards.


The Progression of Serum Prorenin Concentration during Pregnancy

Y Mikami, T Narita, Y Takai, K Baba, H Seki - J Hypertens, 2014
The serum PR concentration was examined using the direct enzyme-linked immunosorbent assay (Innovative Research Inc., Novi, MI, USA) in our center. We performed two assays for 1 sample and calculated the mean...

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Human Prorenin ELISA Kit

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