Human Prolactin ELISA Kit
Prolactin (PRL) is a 199 aa, 23kD peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk.
This human prolactin antigen assay is intended for the quantitative determination of prolactin antigen in human plasma or breast milk. The assay measures total human prolactin in the 0.2-200 ng/mL range. Samples with human prolactin levels above 200ng/mL should be diluted in blocking buffer before use. Normal plasma should not require dilution before use in this assay. A 1:2 to 1:4 dilution for breast milk is suggested to ensure that resulting values fall within the linear range of the assay. Strip well format. Reagents for up to 96 tests.
Human prolactin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinlyated anti-human prolactin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total prolactin present in the samples, is reacted with peroxidase-conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prolactin. Color development is proportional to the concentration of prolactin in the samples.
Detection Target: Prolactin
Range: 0.2 - 200 ng/ml
Sensitivity: 0.0319 ng/ml
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