Human Prekallikrein ELISA Kit
Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma prekallikrein is associated with diabetes and cardiovascular disease.
This human prekallikrein total antigen assay is intended for the quantitative determination of total prekallikrein antigen in human plasma. The assay measures human prekallikrein antigen in the 0.02 10g/ml range. If the unknown is thought to have high prekallikrein levels, dilutions may be made in blocking buffer. Normal human plasma should be applied directly to the plate or at a 1:5 dilution for best results. Strip well format. Reagents for up to 96 tests.
Human prekallikrein will bind to the monoclonal capture antibody coated on the microtiter plate. Prekallikrein and kallikrein will react with the antibody on the plate. After appropriate washing steps, anti-human prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prekallikrein. Color development is proportional to the concentration of total prekallikrein in the samples.
Detection Target: Prekallikrein
Range: 0.02 - 10 ug/ml
Sensitivity: 0.012 ug/ml
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