Human Plasminogen Total ELISA Kit
Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis.
This human plasminogen total assay is for the quantitative determination of total plasminogen and plasmin in human plasma, serum, urine, cell culture media, or tissue extracts. The assay measures plasminogen and plasmin antigens in the 0.5 - 500 ng/ml range. If the unknown is thought to have high plasminogen/plasmin levels, dilutions may be made in a similar biological fluid devoid of plasminogen or in blocking buffer. A 1:50,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human plasminogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
Detection Target: Plasminogen
Range: 0.5 - 500 ng/ml
Sensitivity: 0.069 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Kidney International Reports (2018)
"... For urinary plasmin(ogen) measurement in the present study, archived specimens stored at -80 C were thawed on ice and centrifuged at 10,000 g for 15 minutes at 4 C to remove particulate matter. Specimens were then diluted 10 to 100-fold before plasmin(ogen) measurement via ELISA (Innovative Research, Novi, MI). Although this method does not distinguish active plasmin from plasminogen, results using this assay correlate well with urine plasmin as measured by immunoblot."(1-24-18)
ALBUMINURIA IN KIDNEY TRANSPLANT RECIPIENTS IS ASSOCIATED WITH INCREASED URINARY SERINE PROTEASES AND ACTIVATION OF THE EPITHELIAL SODIUM CHANNELGitte Rye Hinrichs, Jannie Solmunde Michelsen, Rikke Zachar, Ulla Glenert Friis, Per Svenningsen, Henrik Birn, Claus Bistrup, and Boye L Jensen
American Journal of Physiology-Renal Physiology - 24 January 2018
... plasminogen and plasmin concentration) and plasma aldosterone were analyzed using commercial ELISA 122 Kits (Human Plasminogen Total Antigen, IHPLGKT-TOT, Innovative Research, Inc., Novi, Michigan, 123 USA and MS E-5200, Labor Diagnostika Nord GmbH & Co ...(1-20-15)
Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin and urokinase and activation of amiloride-sensitive current in
H Andersen, UG Friis, PBL Hansen, P Svenningsen - Nephrology Dialysis , 2015
... abbreviated: plasmin(ogen), prostasin and urokinase (uPA) were measured using ELISA kits:plasminogen (Cat IHPLGKT-TOT; Innovative Research, Novi, MI, USA); prostasin (Cat ... The study was funded by Strategic Research (Danish Innovation Foundation), The