Human PAI-1 tPA Complex ELISA Kit
Human PAI-1 tPA Complex ELISA Kit is intended for the quantitative determination of the covalent complex of tPA and its inhibitor PAI-1 in biological fluids.issue-type plasminogen activator (tPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and uPA. PAI-1 tPA complex increases during pregnancy, artherosclerosis, sepsis, and are associated with myocardial infarction reoccurrence. High levels of PAI-1 tPA complex in breast cancer cytosols are associated with poor survival. PAI-1 tPA complex levels may also be useful as a prognostic indicator for renal/bladder cancer, multiple organ failure, and stroke. The sensitive quantitative measurement of total human PAI-1 tPA complex antigen in plasma, serum, urine, cell culture media, or tissue extract samples is easily performed with this 96 well strip format ELISA kit.
Concentration of PAI-1 tPA complex in normal human plasma was found to be 2.8 ng/ml. The assay measures PAI-1 tPA complex in the 0.5-200 ng/ml range. Samples with complex levels above 200 ng/ml should be diluted in plasma or similar fluid devoid of PAI-1 or tPA. Human tPA will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of PAI-1 tPA complex in the samples. A standard calibration curve is prepared in PAI-1 depleted plasma using dilutions of purified PAI-1 tPA complex and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.