Human PAI-1 tPA Complex ELISA Kit
Tissue-type plasminogen activator (tPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with uPA and tPA.
Human PAI-1/tPA complex assay is intended for the quantitative determination of the covalent complex of tPA and its inhibitor PAI-1 in human plasma, serum, urine, cell culture media, or tissue extracts. The assay measures PAI-1/tPA antigen in the 0.5 - 100 ng/ml range. If the unknown is thought to have high PAI 1/tPA complex levels, dilutions may be made in plasma devoid of PAI-1 (cat IHPLA-SC-PAI-tPA) or in additional zero unit standard. Strip well format. Reagents for up to 96 tests.
Human PAI-1 in samples will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will react with the capture antibody. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured PAI-1/tPA complex. Excess primary antibody is washed away and bound antibody is then reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of PAI-1/tPA complex. Color development is proportional to the concentration of PAI-1/tPA complex in the samples. Free tPA and PAI-1 will not be detected by this assay.
Detection Target: PAI-1-TPA complex
Range: 0.5 - 100 ng/ml
Sensitivity: 0.03 ng/ml
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