Human PAI-1 total antigen ELISA kit
Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis. The sensitive quantitative measurement of total human PAI-1 antigen in plasma and other biological samples is easily performed with this 96 well strip format ELISA kit. The PAI-1 concentration of normal platelet-free plasma is 21 ng/ml, platelet-rich plasma is 283 ng/ml and serum is 270 ng/ml. The assay measures human PAI-1 in the 0.1-100 ng/ml range. Samples giving human PAI-1 levels above 100 ng/ml should be diluted in PAI-1 depleted plasma before use. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1. Human PAI-1 will bind to the capture antibody coated on the microtiter plate.