Human PAI-1 Total Antigen ELISA Kit
PAI-1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy, sepsis and obesity.
This human plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in human plasma in citrate, EDTA or heparin, serum, cell culture media, tissue lysates and other biological fluids. The assay measures PAI-1 antigen in the 0.25 - 100 ng/ml range. If the unknown is thought to have high PAI 1 levels, dilutions may be made in plasma devoid of PAI 1 or in blocking buffer. A 1:10 to 1:100 dilution for normal plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human PAI-1 will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, polyclonal anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1. Color development is proportional to the concentration of PAI-1 in the samples.
Detection Target: PAI-1
Range: 0.25 - 100 ng/ml
Sensitivity: 0.033 ng/ml
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