Human PAI-1 Activity ELISA Kit For Non Plasma Samples
PAI-1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI-1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI-1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in non-plasma samples including cell culture media or tissue extracts. The assay measures active PAI-1 in the 0.125 - 100 ng/ml range. If the unknown is thought to have high PAI-1 levels, dilutions should be made in blocking buffer. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in samples reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, monoclonal anti-human PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PAI-1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1.
Detection Target: PAI-1
Range: 0.125 - 100 ng/ml
Sensitivity: 0.023 ng/ml
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