Human Cytokine Assay and ELISA Kits

Human Lactoferrin ELISA Kit

Catalog No.: IRKTAH1137


Category: Assay and ELISA Kits

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Product Description

Human Lactoferrin ELISA Kit

Lactoferrin (aka lactotransferrin or growth-inhibiting protein 12) is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main iron binding protein in milk. Lactoferrin has multiple physiological functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primary source of plasma lactoferrin which is increased during inflammation and bacterial infection.

This human lactoferrin assay is intended for the quantitative determination of lactoferrin in human plasma, serum, milk, and cell culture samples. The assay measures total human lactoferrin in the 0.1 - 100 ng/ml range. If the unknown is thought to have high lactoferrin levels, dilutions may be made in blocking buffer. A 1:100-1:500 dilution for normal human plasma or serum and 1:200,000 dilution for breast milk is suggested for best results. Strip well format. Reagents for up to 96 tests.

Human lactoferrin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated anti human lactoferrin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of human lactoferrin and is measured along with the test samples. Color development is proportional to the concentration of total lactoferrin in the samples.

Detection Target: Lactoferrin
Reactivity: Human
Range: 0.1 - 100 ng/ml
Sensitivity: 0.019 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants

S Becker-Dreps, WS Choi, L Stamper, S Vilchez - Journal of the Pediatric , 2015
... where serum samples were separated and then stored at 70C and breast milk samples were stored at 70C. Commercial kits were used to assay breast milk concentrations of lactoferrin (Innovative Research Inc, Novi, MI)...

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Human Lactoferrin ELISA Kit

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