Human IgG3 ELISA Kit
Human IgG3 ELISA kit is designed for detection of human IgG3 in plasma, serum, urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IgG3 in less than 4 hours. A polyclonal antibody specific for human IgG3 has been precoated onto a 96-well microplate with removable strips. IgG3 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for IgG3, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.