Human IgG ELISA Kit
IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.
This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses. The assay measures total human IgG antigen in the 0.2 - 200 ng/ml range. If the unknown is thought to have IgG levels greater than 200ng/ml, dilutions may be made in blocking buffer before use. A 1:1,000,000-1:5,000,000 dilution for normal human plasma is suggested for best results. Strip well format. Reagents for up to 96 tests.
Human IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgG. Color development is proportional to the concentration of IgG in the samples.
Detection Target: IgG
Range: 0.2 - 200 ng/ml
Sensitivity: 0.088 ng/ml
Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Incorporation of IgG Depletion in a Neutralization Assay Facilitates Differential Diagnosis of Zika and Dengue in Secondary Flavivirus Infection Cases
... Percent IgG depletion was calculated by testing serum samples before and after IgG depletion using the 174 quantitative human IgG antigen ELISA kit (Innovative Research Inc., Novi, MI) following the manufacturer's 175 protocol ...(7-2-12)
Direct Phenotypical and Functional Dysregulation of Primary Human B Cells by Human Immunodeficiency Virus (HIV) Type 1 In Vitro
AJ Peris-Barrios, M Muoz-Fernandez, M Pion - PloS one, 2012 - dx.plos.org
Total IgG, IgE and IgA in culture supernatants were measured using a human IgG, IgE and IgA enzyme-linked immunosorbent assay (ELISA) quantitation kit (Innovative Research, Peary Court, Novi, Mi, USA), according to the manufacturer's specifications.