Human IgE Assay and ELISA Kits

Human IgE ELISA Kit

Catalog No.: IHUIGEKT


Category: Assay and ELISA Kits

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Product Description

Human IgE ELISA Kit

IgE is the least abundant immunoglobulin in serum and is predominately involved in the allergy response. IgE binds to allergens and triggers histamine release from mast cells and basophils. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections.

This human immunoglobulin E (IgE) antigen assay is intended for the quantitative determination of total human IgE antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay measures human IgE antigen in the 0.5 - 500 ng/ml range. If the unknown is thought to have high IgE levels, dilutions may be made in blocking buffer. A 1:10 dilution for normal human plasma is suggested for best results.

Human IgE will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgE antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgE. Color development is proportional to the concentration of IgE in the samples. Strip well format. Reagents for up to 96 tests.

Detection Target: IgE
Reactivity: Human
Range: 0.5 - 500 ng/ml
Sensitivity: 0.38 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Direct Phenotypical and Functional Dysregulation of Primary Human B Cells by Human Immunodeficiency Virus (HIV) Type 1 In Vitro

AJ Peris-Barrios, M Muoz-Fernandez, M Pion - PloS one, 2012 - dx.plos.org
Total IgG, IgE and IgA in culture supernatants were measured using a human IgG, IgE and IgA enzyme-linked immunosorbent assay (ELISA) quantitation kit (Innovative Research, Peary Court, Novi, Mi, USA), according to the manufacturer's specifications.

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Human IgE ELISA Kit

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