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Human IgA Assay and ELISA Kits

Human IgA ELISA Kit

Catalog No.: IHUIGAKT

$325

Category: Assay and ELISA Kits

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Product Description

Human IgA ELISA Kit


IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma. IgA in serum is a primarily monomeric 160kDa glycoprotein that initiates defenses against natural infection through interaction with specific receptors and immune mediators. Each monomer consists of two heavy chains and two kappa or lambda light chains. A majority of serum IgA molecules are subclass IgA1 which have longer hinge regions than subclass IgA2.

This Human Immunoglobulin A (IgA) antigen assay is intended for the quantitative determination of total human IgA antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgA subclasses. The assay measures IgA antigen in the 0.1 - 100 ng/ml range. If the unknown is thought to have high IgA levels, dilutions may be made in blocking buffer. A 1:100,000 to 1:800,000 dilution for normal human serum or plasma is suggested for best results.Strip well format. Reagents for up to 96 tests.

Human IgA will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgA antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgA. Color development is directly proportional to the concentration of total IgA in the samples.

Detection Target: IgA
Reactivity: Human
Range: 0.1 - 100 ng/ml
Sensitivity: 0.083 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
(7-2-12)

Direct Phenotypical and Functional Dysregulation of Primary Human B Cells by Human Immunodeficiency Virus (HIV) Type 1 In Vitro


AJ Peris-Barrios, M Muoz-Fernandez, M Pion - PloS one, 2012 - dx.plos.org
Total IgG, IgE and IgA in culture supernatants were measured using a human IgG, IgE and IgA enzyme-linked immunosorbent assay (ELISA) quantitation kit (Innovative Research, Peary Court, Novi, Mi, USA), according to the manufacturer's specifications.

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Human IgA ELISA Kit

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