Human Hemopexin ELISA Kit (Urine, Saliva, Milk, and Cell Culture)
The Human Hemopexin ELISA kit is designed for detection of human hemopexin in urine, saliva, milk and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures hemopexin in less than 4 hours. A polyclonal antibody specific for hemopexin has been pre-coated onto a microplate. Hemopexin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for hemopexin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compoundsin the extracellular compartment. Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which hemopexin is recycled to the circulation (1). Studies indicated that increased hemopexin level associate with minimal change disease (MCD) (2), sporadic Alzheimer's disease (AD) (3), heavy-drinking chronic alcoholics (4), hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria (5).