Human Heat shock proteins ELISA Kit
Human Heat shock proteins ELISA Kit is designed for detection of human HSP47 in plasma, serum, milk, tissue extract, and cell culture lysates. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Hsp47 in less than 5 hours. A polyclonal antibody specific for human Hsp47 has been pre-coated onto a 96-well microplate with removable strips. Hsp47 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Hsp47, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.