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Human Fibrinogen Assay and ELISA Kits

Human Fibrinogen ELISA Kit

Catalog No.: IHFBGNKT

$250

Category: Assay and ELISA Kits

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Product Description

Human Fibrinogen ELISA Kit


Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation. Elevated fibrinogen levels are correlated with cardiovascular disease and atherosclerosis.

This human fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in human plasma and serum. The assay measures fibrinogen antigen in the 3.125-800 ng/ml range. If the unknown is thought to have high fibrinogen levels, dilutions may be made in diluent. It is suggested that normal human plasma samples be diluted 1:100,000 to 1:1,000,000 in diluent, and normal human serum samples be diluted 1:100 in diluent. Strip well format. Reagents for up to 96 tests.

Human fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-human fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

Detection Target: Fibrinogen
Reactivity: Human
Range: 3.125 - 800 ng/ml
Sensitivity: 0.22 ng/ml

Disclaimer: This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

(7-11-16)

Antibody functionalized graphene biosensor for label-free electrochemical immunosensing of fibrinogen, an indicator of trauma induced coagulopathy


W Saleem, C Salinas, B Watkins, G Garvey - Biosensors and , 2016
........ Plasminogen-depleted fibrinogen from human plasma, human plasminogen from fresh human plasma, human fibrinogen ELISA kit, and single donor human whole blood were purchased from Innovative Research (Novi, MI, USA)....

(2-23-09)

A virally inactivated functional growth factor preparation from human platelet concentrates


CY Su, YP Kuo, YC Lin, CT Huang... - Vox ..., 2009 - Wiley Online Library
...Protein determination. Protein concentration was assessed using a Bradford reagent (Sigma) and bovine serum albumin as a standard. Fibrinogen was determined by immunoperoxidase assay using human fibrinogen ELISA kit from Innovative Research (Novi, MI, USA).

(11-27-12)

Deficiency of Growth Factor Midkine Exacerbates Necrotizing Glomerular Injuries in Progressive Glomerulonephritis


H Kojima, T Kosugi, W Sato, Y Sato, K Maeda... - The American journal of ..., 2012 - Elsevier
...The extent of fibrin deposition for each subject, which was indicated as PTAH- or fibrinogen-positive areas, was...were measured using specific enzyme-linked immunosorbent assays (ELISA) according to the instructions of the manufacturer (Innovative Research, Novi,...

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Human Fibrinogen ELISA Kit

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