Human Factor XII ELISA Kit
Human Factor XII ELISA Kit measures total human Factor XII in the 0.1-100 ng/mL range. Samples giving human Factor XII levels above 100ng/mL should be diluted in blocking buffer before use. A 1:1,000 to 1:5,000 dilution for plasma is suggested for best results. The concentration of Factor XII in normal human plasma has been found to be 29 g/mL, with variation among individuals from 15 to 47 g/mL. Another series of studies found values in the 35-40 g/mL range.
Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation.